Multiple retrograde tracing methods compatible with 3DISCO clearing
نویسندگان
چکیده
منابع مشابه
Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion.
Tissue clearing and subsequent imaging of intact transparent tissues have provided an innovative way to analyze anatomical pathways in the nervous system. In this study, we combined a recent 3-dimensional imaging of solvent cleared organ (3DISCO) procedure, light-sheet microscopy, fluorescent retrograde tracer, and Imaris software to 3D map corneal sensory neurons within a whole adult mouse tri...
متن کاملRetrograde axonal tracing with fluorescent markers.
The growth of fluorescence imaging technology and the development of sensitive fluorescent retrograde tracers has provided many new approaches for analyzing neuronal circuits. Fluorescent markers provide unparalleled opportunity for combining axonal tract tracing with techniques such as immunohistochemistry or physiological recording. This unit describes the use of six different fluorescent tra...
متن کاملTracing the Retrograde Route in Protein Trafficking
Retrograde transport, in which proteins and lipids are shuttled between endosomes and biosynthetic/secretory compartments such as the Golgi apparatus, is crucial for a diverse range of cellular functions. Mechanistic studies that explore the molecular machinery involved in this retrograde trafficking route are shedding light on the functions of transport proteins and are providing fresh insight...
متن کاملGENERATION OF MULTIPLE SPECTRUM-COMPATIBLE ARTIFICIAL EARTHQUAKE ACCELEGRAMS WITH HARTLEY TRANSFORM AND RBF NEURAL NETWORK
The Hartley transform, a real-valued alternative to the complex Fourier transform, is presented as an efficient tool for the analysis and simulation of earthquake accelerograms. This paper is introduced a novel method based on discrete Hartley transform (DHT) and radial basis function (RBF) neural network for generation of artificial earthquake accelerograms from specific target spectrums. Acce...
متن کاملIs Multiple Realizability Compatible With Antireductionism?
In a rich and provocative paper, Jaegwon Kim offers an ingenious argument for psychophysical reductionism. I take Kim to be posing a dilemma for anyone who would deny reductionism, viz. that one must either advocate the wholesale reduction of psychology to physical science or one must advocate the sundering of psychology into distinct fields, each one of which is reducible to physical science. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Artificial Cells, Nanomedicine, and Biotechnology
سال: 2019
ISSN: 2169-1401,2169-141X
DOI: 10.1080/21691401.2019.1687493